Learn about protein analysis methods and technologies
Protein biology encompasses both the study of the structure and function of proteins as the primary focus of investigation, and the use of antibodies, proteins, and peptides as tools to purify, detect, and characterize biological systems. Certain methods of protein analysis, such as western blotting and ELISA, have been used routinely in laboratories for many years. Others, such as quantitative protein mass spectrometry, are relatively recent and rapidly developing technologies. New tools and products are continually being developed to advance all aspects of protein biology research.
The purpose of this learning center is to connect scientists (whether new or experienced) to our many resources for learning about protein analysis methods and products.
Topics in protein biology
- Protein Biology Resource Library
- Protein Expression Basics Educational Hub
- Protein Expression Resource List
- Protein Isolation and Purification Information
- Protein Gel Electrophoresis and Western Blotting Education Center
- Protein Assays and Analysis Information
- Protein Mass Spectrometry Information
- Protein Modification and Crosslinking Information
- Antibodies and Proteins Information
Protein sample preparation eLearning course
This modular, animated, and narrated eLearning course was developed to provide a succinct, contextual summary of common laboratory methods and techniques required to achieve optimal results in protein assays and experiments. There are knowledge checks throughout the course to test what you have learned.
Protein expression basics educational hub
An online hub that brings together educational content and tools to enhance your research. Here you can access our protein expression selection guide, educational videos, and other technical resources. For instance, access a curated list of publications that highlights the advantages of our Expi protein expression systems here. Start here if you are new to protein expression or need to refresh your knowledge.
|
|
|
|
Protein expression resource list
Learn about methods and technologies for cell-based or cell-free protein production, including algal, bacterial, mammalian, insect, and yeast expression systems; find vector systems that are easy to use and deliver high protein yields for every downstream application.
|
|
|
Protein isolation and purification
Learn about methods and technologies for efficient cell lysis, protein extraction and fractionation, targeted inhibition of unwanted protease and phosphatase activity, and convenient devices and high performance resins for the purification and clean-up of proteins and antibodies for downstream applications.
|
|
|
Protein gel electrophoresis
Learn about methods and technologies for separation and analysis of proteins by polyacrylamide gel electrophoresis (PAGE). Understand gel types (pouring, precast, gradient, 1D, 2D, etc.), gel and running buffer systems, sample preparation and loading, molecular weight markers (protein ladders), and gel stains.
|
|
|
Western blotting
Learn about methods and technologies for western blot detection and imaging. Understand the reagents and instruments used for the efficient membrane transfer and detection of proteins, including wash and blocking buffers, secondary antibody conjugates, and chemiluminescent, colorimetric or fluorescent detection substrates.
|
|
|
Protein assays and analysis
Learn about methods and technologies for identifying and measuring proteins. Understand protein quantitation reagents, ELISA and other immunoassays, protein interaction and activity assays, enzyme reporter assays, protein microarrays, and immunoprecipitation and pull-down tools.
|
|
|
Protein mass spectrometry
Learn about methods and technologies for peptide and protein analysis by high performance liquid chromatography and mass spectrometry (LC/MS). Understand sample prep tools, mass and isotope labeling strategies for quantitative proteomics, and the basics of MS data and analysis.
|
|
|
Protein modification and crosslinking
Learn about methods and technologies for protein labeling, modification, and crosslinking. Understand the chemical functional groups and reaction chemistries required to crosslink and conjugate proteins to one another; to label proteins with biotin, PEG, or fluorescent dyes; or to reduce, cleave, and fragment proteins for use in assays.
|
|
|
Antibodies and proteins
Learn about methods and technologies for preparing and using antibodies and recombinant proteins in protein analysis techniques. Understand how specific primary and secondary antibodies are produced, screened, purified, and modified to enable their use in a myriad of protein detection (identification and measurement) applications.
|
|
|
Protein Biology Resource Library
Browse protein biology learning center content by collection (resource type) — protein methods articles, product application notes, selection guides, webinars, technical handbooks, etc.
Resource library features
Methods article (videos)
Videos that demonstrate each step in the western blot workflow, including SDS-PAGE, transfer, blocking, probing with antibodies, chemiluminescent detection, and stripping the blot.
Methods article
A review of the enzyme-linked immunsorbant assay (ELISA), including formats (sandwich), detection methods (direct, indirect), plate coating techniques, antibodies and other probes, buffers, and kits.
Methods article
Strategies for proteomic analyses, including those for relative quantitation (SILAC, ICAT, ICPL and isobaric tags) and for absolute quantitation (via SRM), and both labeled and label-free techniques.
For Research Use Only. Not for use in diagnostic procedures.
