Immunofluorescence Imaging

For critical research, you need reliable protein detection, and we provide a complete suite of labeling tools for any level of target abundance. Our fluorescent products portfolio is the most peer-referenced in life science research.

Check out this simple guide and see which tool is right for your protein.

  • High-abundance target—primary conjugate, no amplification needed
  • Medium- to high-abundance target—secondary conjugate, modest amplification
  • Medium- to low-abundance target—streptavidin conjugate, elevate the signal
  • Low-abundance target—enzyme amplification, complex process for maximum signal enhancement

Immunofluorescence selection guide

 

High-abundance target

e.g., tubulin

Medium-abundance target

e.g., golgi or mitochondria

Low-abundance target

e.g., receptors or cell junction

 

Labeled primary

 

Secondary
detection

 

Streptavidin

 

TSA
technology

Optimum signal for brightness/sensitivity Signal too strong Signal too weak

Primary antibodies

Primary antibodies   Going direct with your choice of label has never been easier.
We offer a wide variety of primary antibodies against key signaling proteins, cellular markers, and organelles, as well as cell junctions and CD markers. Primary antibodies provide direct detection for high-abundance targets, and high target specificity when using amplified detection methods.

Secondary antibodies

Secondary antibodies   Secondary antibodies with a choice of dyes provide that extra signal and assay flexibility.
We offer access to some of the most cited secondary antibodies in life science research, including Alexa Fluor? dye–labeled probes and Qdot? conjugates, as well as classic dyes in a wide range of colors.

Streptavidin

Streptavidin   Streptavidin conjugates with a choice of labels boost your signal from low-abundance targets.
For improved detection sensitivity, streptavidin-based amplification techniques are widely used in fluorescence imaging to detect primary and secondary antibodies, ligands, and toxins, or DNA probes for in situ hybridization.

TSA technology

TSA? technology   Simple amplification protocols with a choice of wavelengths for rare targets.
With Invitrogen? SuperBoost? tyramide signal amplification technology, you can see and measure low-abundance targets that are not detectable by conventional means. Tyramide-based signal amplification provides superior sensitivity without compromising resolution.

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