Immunofluorescence Imaging

For critical research, you need reliable protein detection, and we provide a complete suite of labeling tools for any level of target abundance. Our fluorescent products portfolio is the most peer-referenced in life science research.

Check out this simple guide and see which tool is right for your protein.

High-abundance target—primary conjugate, no amplification needed
Medium- to high-abundance target—secondary conjugate, modest amplification
Medium- to low-abundance target—streptavidin conjugate, elevate the signal
Low-abundance target—enzyme amplification, complex process for maximum signal enhancement

Immunofluorescence selection guide

	High-abundance target e.g., tubulin	Medium-abundance target e.g., golgi or mitochondria	Low-abundance target e.g., receptors or cell junction
Labeled primary
Secondary detection
Streptavidin
TSA technology

Optimum signal for brightness/sensitivity

Signal too strong

Signal too weak

Primary antibodies

Going direct with your choice of label has never been easier.
We offer a wide variety of primary antibodies against key signaling proteins, cellular markers, and organelles, as well as cell junctions and CD markers. Primary antibodies provide direct detection for high-abundance targets, and high target specificity when using amplified detection methods.

Find out more about primary antibodies

Secondary antibodies

Secondary antibodies with a choice of dyes provide that extra signal and assay flexibility.
We offer access to some of the most cited secondary antibodies in life science research, including Alexa Fluor? dye–labeled probes and Qdot? conjugates, as well as classic dyes in a wide range of colors.

Find out more about secondary antibodies

Streptavidin

Streptavidin conjugates with a choice of labels boost your signal from low-abundance targets.
For improved detection sensitivity, streptavidin-based amplification techniques are widely used in fluorescence imaging to detect primary and secondary antibodies, ligands, and toxins, or DNA probes for in situ hybridization.

Find out more about imaging with streptavidin

TSA technology

Simple amplification protocols with a choice of wavelengths for rare targets.
With Invitrogen? SuperBoost? tyramide signal amplification technology, you can see and measure low-abundance targets that are not detectable by conventional means. Tyramide-based signal amplification provides superior sensitivity without compromising resolution.