The EVOS Onstage Incubator option (compatible with the M7000 and M5000 systems) allows imaging and analysis of live cells without removing them from the incubator. This enables precise control of temperature, humidity, and three gases for time-lapse imaging of live cells under both physiological and non-physiological conditions. Environmental settings and image acquisition parameters are all seamlessly integrated into the software interface, creating a high-performance inverted imaging system with unmatched flexibility, ease of use, and optics for demanding time-lapse fluorescence imaging experiments.
Together, the EVOS M7000 or M5000 Imaging System and EVOS Onstage Incubator let researchers observe changes in cellular morphology over time and quantify cellular health, function, and activity under controlled physiological conditions. Such temporal kinetic data at the cellular level is difficult to obtain using traditional imaging solutions.
|Compatible vessels||Well plates, 35 mm dishes, T25 Flasks|
|Temperature range||Ambient to 40°C|
|CO? % range||0 to 20%|
|O2% range||0 to 21% (Ambient)|
|Humidity range||>80% relative humidity at 37°C|
25 x 19 x 3.7 cm (Environment chamber)
37 x 16 x 20 cm (Control unit)
1.5 kg (Environment chamber)
10 kg? (Control unit)
Conventional plastic plates can generate optical problems or even autofluorescence. For optimal results, we recommend choosing dishes, plates, and vessel holders designed specifically for live-cell imaging
Many cell culture media are carbonate buffered and, once removed from the incubator, the relatively lower CO2 concentration of air will cause an increase in the medium pH within an hour. We have developed an optically clear, physiological solution (Live Cell Imaging Solution, or LCIS) that maintains physiological pH and osmolarity for up to 4 hours at ambient atmosphere and temperature. This is especially useful for imaging outside of an incubator, but can also help to maintain pH while using the EVOS Onstage Incubator.
For longer-term imaging, particularly in a microscope-stage incubator, we offer a clear cell culture medium, Gibco FluoroBrite DMEM, that is based on DMEM to help preserve cell health while also providing low background fluorescence (comparable to that of phosphate-buffered saline and 90% lower than standard phenol red–free DMEM).
For Research Use Only. Not for use in diagnostic procedures.