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                Shop All PCR Equipment and Supplies

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                SYBR™ Select Master Mix for CFX Applied Biosystems™

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                Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Select Master Mix for CFX and added additional capabilities for your gene expression analysis.

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                SYBR® Select Master Mix for CFX is a superior and cost-effective solution for your real-time PCR applications.

                • Specific—minimize primer-dimer and non-specific amplification
                • Reproducible and sensitive—consistent amplification across a wide dynamic range
                • Bright—contains SYBR® GreenER™ dye for maximum brightness
                • Carry-over contamination control—contains heat-labile UDG
                • For use with the Bio-Rad CFX96™ Touch or CFX384™ Touch systems
                • Standard and fast cycling parameters

                Formulated for Maximum Specificity
                SYBR® Select Master Mix for CFX contains all the components needed for your real-time PCR reactions, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

                Obtain Reproducible Results Across a Wide Dynamic Range
                SYBR® Select Master Mix for CFX is specially formulated to provide robust results from 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).

                Contains SYBR® GreenER™ Dye and Heat-labile UDG
                • SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye, resulting in brighter signals
                • Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

                View performance data.

                For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

                Maxima SYBR Green/ROX qPCR Master Mix (2X) Thermo Scientific™

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                Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Maxima SYBR Green qPCR Master Mix and added additional capabilities for your gene expression analysis.

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                Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

                Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

                Highlights

                SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
                Sensitivity—detects low copy number targets
                Wide linear range—accurate quantification across 9 orders of magnitude
                Reproducibility and convenience—ready-to-use 2x master mix

                Applications

                • Gene expression
                • siRNA validation
                • Genotyping
                • Pathogen detection

                Includes

                Maxima SYBR Green/ROX qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.

                Related Products
                Maxima SYBR Green/ROX qPCR Master Mix (2X)
                Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
                Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

                MagMAX™ Viral/Pathogen Binding Beads Applied Biosystems™

                These binding beads are included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42352, A42356, A42357, and A42358). They are made available separately for applications that require more binding beads than are provided in the kit.

                DreamTaq™ Buffer (10X) Thermo Scientific™

                Thermo Scientific™ 10X DreamTaq™ Buffer is specifically optimized for PCR using DreamTaq™ DNA Polymerase and DreamTaq™ Hot Start DNA Polymerase. The buffer contains both KCl and (NH4)2SO4 to provide high specificity of primer annealing at a wide range of MgCl2 concentrations. 10X DreamTaq buffer includes 20 mM MgCl2.

                TaqMan™ Gene Expression Cells-to-CT™ Kit Invitrogen™

                The TaqMan® Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification. This kit saves time and offers a simple workflow that is suitable for a few samples or can be easily incorporated into automated, high-throughput applications.

                The TaqMan® Gene Expression Cells-to-CT Kit is:

                • Complete—optimized workflow includes cell lysis reagents with gDNA removal, RT enzyme mix, buffer, and new TaqMan® Gene Expression Master Mix
                • Fast—7-minute sample prep, including DNase treatment, at room temperature
                • Easy—lyse samples in a tube or directly in culture plates
                • Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
                • Efficient—contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples

                Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan® Gene Expression Cells-to-CT™ Kit contains reverse transcription (RT) reagents for cDNA synthesis and TaqMan® Gene Expression Master Mix for real-time PCR analysis. TaqMan® primer⁄probe sets are sold separately.

                Simple 7-minute sample preparation: part of a complete workflow
                Whether you are using plates or tubes, the TaqMan® Gene Expression Cells-to-CT™ Kit, which uses the simple 7-minute sample preparation procedure outlined (see figure), is designed for 10–100,000 cultured cells⁄sample. Cells are washed in PBS and lysed in solution for 5 minutes at room temperature; DNase treatment can be performed simultaneously. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The lysates are now ready for reverse transcription or storage at –20°C. Because samples can be processed directly in culture wells (96 or 384 wells), sample handling and the potential for sample loss or transfer error are minimized, facilitating rapid, high-throughput processing. Unlike old-fashioned, multi-step RNA isolation protocols, no heating, washing, or centrifugation steps are required. The kit greatly simplifies a laborious 30–60 minute process and reduces it to 7 minutes.

                The TaqMan® Gene Expression Cells-to-CT™ Kit workflow enables unsurpassed gene expression evaluation with any of the >700,000 TaqMan® Gene Expression Assays. This kit has been extensively tested for specificity with a broad selection of TaqMan® Gene Expression Assays and shows performance equivalent to that obtained with purified RNA (see figure).

                Achieve unsurpassed performance and sensitivity
                Unlike some competitor kits that limit the amount of lysate in the RT reaction to 5%, the TaqMan® Gene Expression Cells-to-CT™ Kit can accommodate 45% of the total RT reaction volume as cell lysate. Additionally, cDNA can comprise up to 45% of the real-time PCR reaction volume. The large lysate volume in the optimized RT reaction, along with the large cDNA volume in the subsequent real-time PCR using the TaqMan® Gene Expression Master Mix, lead to maximum sensitivity. The master mix amplifies the target precisely and accurately, enabling the detection of small quantities of target, such as transcripts expressed at low levels.

                The ability to detect limited target quantities was tested by mixing a constant number of human cells with various numbers of mouse cells. The cell mixtures were prepared using the TaqMan® Gene Expression Cells-to-CT™ and assayed for a mouse-specific and human-specific gene. Comparative data were generated in the same manner with RNA purified by traditional methodology. The data show that RNA from as few as 10 mouse cells was detectable in a background of RNA from 10,000 human cells (see figure). The ability of the TaqMan® Gene Expression Cells-to-CT™ Kit to detect relative low abundance transcripts was equivalent to that of purified RNA; at low levels, it was superior.

                Additionally, the performance of the TaqMan® Gene Expression Cells-to-CT™ Kit was compared to competitor lysate kits and to purified RNA. Inputs of 100–100,000 cells⁄lysis reaction were examined. The sensitivity of the TaqMan® Cells-to-CT™ Kit protocol was equivalent to that obtained with purified RNA, and it surpassed competitor lysates (see figure). Furthermore, the lack of inhibition at high cellular inputs and the low variation among technical replicates demonstrate the reliability of this approach for gene expression studies using cultured cells.

                Proven performance, proven together
                All components of the TaqMan® Gene Expression Cells-to-CT™ Kit have been optimized for consistent and reliable performance. This removes the guesswork involved in assembling separate sample preparation, RT, and real-time PCR kits. And the TaqMan® Gene Expression Cells-to-CT™ Kit has been validated with TaqMan® Gene Expression Assays for added quality assurance.

                Accessory product
                The TaqMan® Cells-to-CT™ Control Kit was designed for use with the TaqMan® Gene Expression Cells-to-CT™ Kit. This kit contains an endogenous control (ACTB) to normalize for sample loading variability and an artifical XenoRNA™ control and corresponding TaqMan® Gene Expression Assay to monitor the effects of PCR inhibition.

                5' Mod , Alexa Fluor 546 , 200 nmol Invitrogen™

                Use this tool to design a custom DNA oligonucleotide, with any of a variety of 3' and 5' modifications, at scales ranging from 25 nmole to 10 µmole. Select desired purification method (normal or reversed-phase chromatography, HPLC, or PAGE).

                Maxima Probe/ROX qPCR Master Mix (2X) Thermo Scientific™

                **********************
                Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Maxima Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.

                **********************
                Thermo Scientific Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.

                Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (see Reference 1). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.

                Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.

                Highlights

                • Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
                • Sensitivity—detects low copy number targets
                • Wide linear range—accurate quantification across 9 orders of magnitude
                • Reproducibility and convenience—ready-to-use 2X master mix

                Applications

                • Gene expression
                • siRNA validation
                • Genotyping
                • Pathogen detection

                Includes
                Maxima Probe/ROX qPCR Master Mix (2X) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.

                Related Products
                Maxima Probe/ROX qPCR Master Mix (2X)
                Maxima Probe qPCR Master Mix (2X), with separate ROX vial

                MagMAX™ Viral/Pathogen Proteinase K Applied Biosystems™

                This Proteinase K is included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42352, A42356, A42357, and A42358). It is made available separately for applications that require more Proteinase K than is provided in the kit.

                SYBR™ Green PCR Master Mix

                Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems® SYBR® Green PCR Master Mix combines SYBR® Green I dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

                • Premixed components stored at 2–8°C significantly reduce assay setup time
                • SYBR® Green I dye detects double-stranded DNA, so specific probes are not required
                • AmpliTaq Gold® DNA polymerase minimizes nonspecific product formation to achieve superior performance
                • dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
                • Proprietary buffer enhancements ensure performance and reliability

                Maximum Flexibility and Convenience
                Applied Biosystems® SYBR® Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan® probes are required. SYBR® Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR. The hot-start enzyme AmpliTaq Gold® DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity. Passive Internal Reference 1 is provided to normalize non-PCR–related fluorescence fluctuations. This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation. SYBR® Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.

                Alternative product
                Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis.

                TaqMan™ Gene Expression Master Mix Applied Biosystems™

                Get precise and reliable real-time qPCR quantification over nine logs of linear dynamic range with TaqMan Gene Expression Master Mix. TaqMan Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend. TaqMan Gene Expression Master Mix has been validated with TaqMan gene expression assays and Applied Biosystems real-time systems, providing precise quantification for a variety of gene expression–based real-time qPCR applications, including:
                ? Rare transcript detection
                ? Gene expression analysis
                ? Pathogen detection and viral load quantification
                ? Co-amplification of two targets
                ? Specific detection of gene family members

                TaqMan Gene Expression Master Mix contains AmpliTaq Gold DNA Polymerase, UP (Ultra Pure), for hot start activation and improved detection of bacterial targets, a blend of dNTPs with dTTP/dUTP and uracil-DNA glycosylase (UDG) to minimize carry-over PCR contamination, and a passive internal reference based on proprietary ROX dye for superb precision on Applied Biosystems real-time PCR instruments.

                Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Gene Expression Master Mix and added additional capabilities for your gene expression analysis.

                EXPRESS One-Step SYBR™ GreenER™ Kit, universal Invitrogen™

                EXPRESS One-Step SYBR GreenER kits are compatible with fast-cycling instruments for your SYBR-based qPCR experiments. This kit comes with a separate tube of ROX reference dye. EXPRESS One-Step SYBR GreenER SuperMix kits with premixed ROX reference dye are also available.

                This kit provides:
                • Fast-activating, antibody-mediated Platinum™ Taq DNA Polymerase
                • The One-Step SuperScript™ III Reverse Transcriptase for one-step qRT-PCR
                • SYBR GreenER dye
                • UDG carryover protection—uracil-DNA-glycosylase (UDG)/dUTP to reduce carryover contamination

                Custom Primer Invitrogen™

                Use this tool to design a custom DNA oligonucleotide, with any of a variety of 3' and 5' modifications, at scales ranging from 25 nmole to 10 µmole. Select desired purification method (normal or reversed-phase chromatography, HPLC, or PAGE).

                Eureka™ Ovine Parentage Panel-163 plex Applied Biosystems™

                Accelerate genetic improvement in sheep by increasing pedigree accuracy

                Eureka™ Ovine Parentage Panel is a comprehensive parentage panel for sheep, providing superior power to accurately verify parentage. It was developed for use in globally diverse breeds of sheep. The panel covers 163 parentage SNPs for high accuracy, and includes a subset of 109 SNPs for use with North American breeds.

                Eureka Ovine Parentage Panel offers an affordable, high-throughput, and robust targeted genotyping by sequencing (GBS) technology to parentage studies, increasing the accuracy of ovine breeding programs. The availability of over 3,000 barcodes enables processing of over 3,000 samples in a single sequencing run for fast turnaround time. The low marker-density panel ensures accurate interrogation of all the ovine parentage markers in all regions of the genome. The 163-SNP panel can be customized to include additional markers for enhancing the accuracy and value of the panel.

                Highlights of Eureka Ovine Parentage Panel:

                Highly informative and well-characterized markers - covers 163 parentage SNPs for higher accuracy, with minimal overlap of highly informative SNP markers.
                Ideal for North American breeds - contains a subset of 109 SNPs for use with North American breeds.
                Robust marker selection - ensures genotypes through our internal validation processes.
                Affordable - use with globally diverse breeds and achieve high overall genotyping efficiency and economy of scale.
                Automated analysis - Eureka Analysis Suite manages samples, ensures next-generation sequencing (NGS) index combination integrity, and enables genotyping data analysis with a single software package.

                ViiA™ 7 Array Card RNaseP Verification Kit Applied Biosystems™

                The ViiA™ 7 System TaqMan® Low Density Array Instrument Verification RNase P kit is used to validate the performance of the Applied Biosystems® ViiA™ 7 Real-Time PCR Systems with the TaqMan® Low Density Array Block Upgrade.

                The ViiA™ 7 System TaqMan® Low Density Array Instrument Verification RNase P kit contains an empty TaqMan® Low Density Array and 8 vials of RNase P detection reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each vial contains premixed reagents (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye-labeled probe) and template.

                The ViiA™ 7 System TaqMan® Low Density Array Instrument Verification RNase P kit must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems® recommends that the performance of your instrument be verified every six months.

                Custom Primer Invitrogen™

                Use this tool to design a custom DNA oligonucleotide, with any of a variety of 3' and 5' modifications, at scales ranging from 25 nmole to 10 µmole. Select desired purification method (normal or reversed-phase chromatography, HPLC, or PAGE).
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